publications

2024

1. Mitochondrial efficiency determines Crabtree effect across yeasts

   Julius Battjes, Pranas Grigaitis, Milou Hoving, and 7 more authors

   bioRxiv, 2024

   Abs

   Under excess glucose conditions, many yeasts switch from high-yield respiratory metabolism to low-yield fermentation, a phenomenon called the Crabtree effect in yeast, or the Warburg effect in mammalian cells. Cellular constraints and limited resources are generally believed to govern the metabolic strategies of cells to adapt to environmental conditions, but which constraints drive this switch is still under debate. Here we study the Crabtree-negative, fully respiratory yeast Pichia kluyveri and compare it to the Crabtree-positive yeast Saccharomyces cerevisiae from a resource allocation perspective. By integrating quantitative physiology and proteomics into whole-cell proteome-constrained models, we find that the Crabtree effect is determined by the composition and catalytic efficiency of the electron transport chain. We find that the subsequent proteome efficiency of respiration versus fermentation varies between these species. The variation in parameters and composition of the respiratory machinery likely reflects the evolutionary and ecological history of these yeast species. This study advances our understanding of the role of proteome constraints and proteome efficiency in governing cellular metabolism of yeasts, and that of eukaryotic cells at large.Competing Interest StatementThe authors have declared no competing interest.

2023

1. Elevated energy costs of biomass production in mitochondrial respiration-deficient Saccharomyces cerevisiae

   Pranas Grigaitis, Samira L Bogaard, and Bas Teusink

   FEMS Yeast Research, 2023

2022

1. Transcription activation is enhanced by multivalent interactions independent of phase separation

   Jorge Trojanowski, Lukas Frank, Anne Rademacher, and 3 more authors

   Molecular Cell, 2022
2. Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies

   Ibrahim E Elsemman, Angelica Rodriguez Prado, Pranas Grigaitis, and 8 more authors

   Nature Communications, 2022
3. Use and limitations of genome-scale metabolic models in food microbiology

   Vincent Somerville, Pranas Grigaitis, Julius Battjes, and 2 more authors

   Current Opinion in Food Science, 2022
4. A computational toolbox to investigate the metabolic potential and resource allocation in fission yeast

   Pranas Grigaitis, Douwe AJ Grundel, Eunice Pelt-KleinJan, and 5 more authors

   mSystems, 2022
5. An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability

   Pranas Grigaitis, and Bas Teusink

   FEBS Letters, 2022

2021

1. Protein cost allocation explains metabolic strategies in Escherichia coli

   Pranas Grigaitis, Brett G Olivier, Tomas Fiedler, and 3 more authors

   Journal of Biotechnology, 2021
2. Lysosome-targeted photodynamic treatment induces primary keratinocyte differentiation

   Neringa Daugelaviciene, Pranas Grigaitis, Liepa Gasiule, and 3 more authors

   Journal of Photochemistry and Photobiology B: Biology, 2021

2020

1. miRNA target identification and prediction as a function of time in gene expression data

   Pranas Grigaitis, Vytaute Starkuviene, Ursula Rost, and 3 more authors

   RNA biology, 2020

2019

1. Exogenous interleukin-1αsignaling negatively impacts acquired chemoresistance and alters cell adhesion molecule expression pattern in colorectal carcinoma cells HCT116

   Pranas Grigaitis, Violeta Jonusiene, Vilmante Zitkute, and 3 more authors

   Cytokine, 2019
2. Epistatic analysis of the contribution of rabs and kifs to CATCHR family dependent golgi organization

   Shijie Liu, Waqar Majeed, Pranas Grigaitis, and 4 more authors

   Frontiers in Cell and Developmental Biology, 2019

2015

1. The role of interleukin-8 (CXCL8) and CXCR2 in acquired chemoresistance of human colorectal carcinoma cells HCT116

   Daiva Dabkeviciene, Violeta Jonusiene, Vilmante Zitkute, and 4 more authors

   Medical Oncology, 2015